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CyFlow™ ARHGEF4 PE

CyFlow™ ARHGEF4 PE
Application: Flow cytometry
Clonality: monoclonal
Target Species: Human
Regulatory Status: RUO
Laser: Blue, Green, Yellow
Source Species: Mouse
Format/Fluorochrome: PE
Excitation Maximum: 496 nm, 565 nm
Emission Maximum: 576 nm
Isotype: IgG1
Field of Interest: Signaling
Antigen: ARHGEF4
Clone: ARHGEF-08
Order number: BV459562
217,72  EUR *

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Concentration Unit mg/mL Concentration 0,1 Quantity 0.1 mg Volume 1.0 mL... more
Product information "CyFlow™ ARHGEF4 PE"
Application: Flow cytometry
Clonality: monoclonal
Target Species: Human
Regulatory Status: RUO
Laser: Blue, Green, Yellow
Source Species: Mouse
Format/Fluorochrome: PE
Excitation Maximum: 496 nm, 565 nm
Emission Maximum: 576 nm
Isotype: IgG1
Field of Interest: Signaling
Antigen: ARHGEF4
Clone: ARHGEF-08
Concentration Unitmg/mL
Concentration0,1
Quantity0.1 mg
Volume1.0 mL
ImmunogenRecombinant fragment of human ARHGEF4 (amino acids 143-271)
Background InformationARHGEF4 (Rho guanine nucleotide exchange factor 4), also known as ASEF1 (Adenomatous polyposis coli - stimulated guanine nucleotide exchange factor 1) is an approximately 80 kDa cytoplasmic protein important for growth factor-mediated regulation of cell morphology and migration. Besides N-terminal adenomatous polyposis coli (APC)-binding region (ABR) it contains Dbl homology (DH), Pleckstrin homology (PH) and SH3 domains. The SH3 domain inhibits GEF activity of ARHGEF4 by intramolecular interaction with the DH domain, whereas binding of APC stimulates the GEF activity. Activated ARHGEF4 stimulates the small GTPase Cdc42, which leads to decreased cell-cell adherence and enhanced cell migration.
UsageThe reagent is designed for Flow Cytometry analysis. Working concentrations should be determined by the investigator.
Storage BufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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