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CyFlow™ IgG1 Alexa Fluor™ 488

CyFlow™ IgG1 Alexa Fluor™ 488
Clonality: monoclonal
Regulatory Status: RUO
Laser: Blue
Source Species: Mouse
Isotype: IgG1
Format/Fluorochrome: Alexa Fluor™ 488
Excitation Maximum: 495 nm
Emission Maximum: 519 nm
Antigen: IgG1 Isotype Control
Clone: MOPC-21
Application: Flow cytometry (Isotype control)
Field of Interest: Isotype controls
Order number: AD892927
213,50  EUR *

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The specificity of staining by monoclonal antibodies to target antigens should be verified by... more
Product information "CyFlow™ IgG1 Alexa Fluor™ 488"
The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MOPC-21, generated against an undefined antigen, does not react specifically with rat and human samples, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG1 molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.
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