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Test kit for the specific detection of living Brettanomyces bruxellensis cells in wine and pre-mature wine sample. The specifically programmed VIT® (VIT® = vermicon identification technology) gene probes bind to target structures within the corresponding living yeast in the wine sample material and deliver maximum specificity and speed for the detection. The reagent has been specifically adapted for selected configurations of the flow cytometer CyFlow Cube V2m.