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CyFlow™ Beta-catenin Purified

CyFlow™ Beta-catenin Purified
Application: Flow cytometry, Immunocytochemistry, Immunoprecipitation, Western blot
Target Species: Hamster, Human, Mouse
Clone: EM-22
Field of Interest: Adaptor proteins, Cytoskeleton, Transcription
Clonality: monoclonal
Isotype: IgG1
Format/Fluorochrome: Purified
Source Species: Mouse
Regulatory Status: RUO
Antigen: β-catenin
Order number: BP858553
184,33  EUR *

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Concentration Unit mg/mL Concentration 1 Quantity 0.1 mg Volume 0.1 mL... more
Product information "CyFlow™ Beta-catenin Purified"
Application: Flow cytometry, Immunocytochemistry, Immunoprecipitation, Western blot
Target Species: Hamster, Human, Mouse
Clone: EM-22
Field of Interest: Adaptor proteins, Cytoskeleton, Transcription
Clonality: monoclonal
Isotype: IgG1
Format/Fluorochrome: Purified
Source Species: Mouse
Regulatory Status: RUO
Antigen: β-catenin
Concentration Unitmg/mL
Concentration1
Quantity0.1 mg
Volume0.1 mL
ImmunogenRecombinant human β-catenin
Background Informationβ-catenin is a multifunctional protein involved both in cell adhesion and in activation of transcription. Calcium-dependent intercellular adhesion transmembrane glycoprotein E-cadherin interacts by its cytoplasmic domain with reciprocally bound α, β and γ catenin. β-catenin links this complex through α-actinin to the cytoskeleton. Functional cadherin-catenin system is important for invasiveness of tumor cells. β-catenin level in cytoplasm is controlled by glycogen synthase kinase-3 β. When activity of this kinase is blocked (e.g. by excessive stimulation of Wnt signaling pathway), hypophosphorylated stable form of β-catenin accumulates in the cytoplasm, translocates to the nucleus and activates transcription of genes including those that are involved in cell cycle control. As a result, cell division and neoplastic transformation are promoted.
Storage BufferThe reagent is provided in phosphate buffered saline (PBS) solution, pH ≈7.4, containing 0.09% (w/v) sodium azide.
StorageAvoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
StabilityDo not use after expiration date stamped on vial label.
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